Supplemental Data
Supplemental Data for
Hu et al., Cancer Cell 13, pp. 394-406
- Document S1. Supplemental Experimental Procedures, Four Supplemental Tables, and Eight Supplemental Figures
- Table S5. Comparison of SAGE Tags that Are Statistically Significantly Differentially Expressed between ITGB6+
and MUC1+ Cells to SAGE Libraries Prepared from Human Breast Myoepithelial and Epithelial Cells
All SAGE libraries
were normalized to total tag count of 100,000. Tags only present in ITGB6+ or MUC1+ cells from MCFDCIS xenografts and in none
of the libraries prepared from primary human tissue were removed. Tags statistically significantly differentially (p <
0.05) expressed between ITGB6+ and Muc1+ cells were linked to “MYOEP” (CD10+ myoepithelial cells purified from normal breast
tissue or DCIS) and “EPI” (BerEP4+ epithelial cells purified from normal or cancerous breast tissue) samples. Two-tailed Student's
t test (T-test) and Wilcox Rank Sum test (Wilcox test) were performed between the “MYOEP” and “EPI” groups, and the ratio
of the mean of “MYOEP” and “EPI” groups was calculated (Ratio MYOEP/EPI). If the ratio of ITGB6+/MUC1+ and MYOEP/EPI are in
the same and opposite directions, then the tag is labeled as “+” and “?”, respectively (Direction). To select for DMYOEP-specific
genes, the ratio of the mean of “DMYOEP” and “NMYOEP” groups was also calculated (Ratio D/N). Tag sequence, normalized tag
counts, p value for the difference between ITGB6+ and MUC1+ libraries, T-test, Wilcox test, Mean MYOEP, Mean EPI, Ratio MYOEP/EPI,
Direction, Mean DMYOEP, MEAN NMYOEP, Ratio D/N, UniGene ID, Gene symbol, Gene description, and Cellular localization are listed.
In the SAGE library: N, normal; D, DCIS; and I, invasive ductal carcinoma.
